| Article DOI |
10.1021/ja029029o |
| Data DOI |
N/A |
| Internal ID |
10.1021/ja029029o/1 |
| Membrane composition (molar fraction) |
| SDPC |
1-stearyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine |
1 |
|
| Solution composition |
Pure water
|
| Temperature (K) |
303.15 |
| Total hydration |
50 (mass %) |
| pH |
UNKNOWN |
| Reagent sources |
| SDPC |
(d3518:0)-SDPC was synthesized by Avanti Polar lipids. |
| water |
Carefully degassed, deuterium-depleted water or D2O (Cambridge Isotope Laboratories, Woburn, MA) |
|
| Sample protocol |
lipid/water dispersions were homogenized by stirring and centrifugation and then packed |
| Show on GitHub |
experiments/OrderParameters/10.1021/ja029029o/1 main |
| Name |
Value |
| DATE |
2003-05-21 05:48:00
|
| ADDITIONAL_MOLECULES |
| Key |
Value |
| DTPA |
diethylenetriaminepentaacetic acid, 1 mM |
| BHT |
2,6-di-tert-butyl-4-methylphenol, 1:150 (bht:lipid) |
|
| NMR |
| Key |
Value |
| T_RF_HEATING |
measured |
| INSTRUMENT |
Bruker DMX500 and DMX300, 4mm MAS probe |
| METHOD |
2H:QE, 13C:INVREC/CP |
| SIGN_MEASURED |
NONE |
| DETAILS |
Deuterium NMR spectra were observed at 46.1 MHz using a quadrupolar echo pulse sequence (d1-90°x-τ-90°y-acquire)
with a repetition time d1=0.5s, a 2.7-μs 90° pulse, a delay time τ=50μs, and a spectral width of 200kHz.
T1 were measured at a MAS spinning frequency of 10 kHz by the inversion−recovery pulse sequence (d1-180°-τ-90°-acquire),
a delay time d1=11.5s, a 5.5-μs 90° pulse, and a spectral width of 27.7kHz. The experiments were conducted with 50 kHz
of proton noise decoupling during data acquisition.
The 13C NMR cross-polarization experiments were conducted at a MAS spinning frequency of 5kHz using a shaped spin-lock pulse
that increments the carbon spin-lock field strength from 20kHz to 25kHz in 10 steps. 24 The spin-lock field strength for
1H resonances was adjusted to achieve maximum signal intensity using an adamantane sample. Signal intensity of lipid samples
was recorded as a function of contact time over the range from 100 μs to 300 ms.
Order parameter for 13C-1H was estimated form CP magnetization transfer as described in the manuscript.
|
|