Experiment OP - 10.1021/bi00595a029/1

Article DOI 10.1021/bi00595a029
Data DOI N/A
Internal ID 10.1021/bi00595a029/1
Membrane composition (molar fraction)
DPPC 1,2-dipalmitoyl-sn-glycero-3-phosphocholine 1
Solution composition
Compound Mass %
ACE 0.59
SOD 0.23
Temperature (K) 331
Total hydration 50 (mass %)
pH 5.7
Reagent sources
DPPC Synthesized locally, specifically deuterated at the choline alpha (+NCD2CH2-DPPC), beta (+NCH2CD2-DPPC), and gamma ((CD3)3N+-DPPC) segments
water Unknown
EDTA Unknown
acetic_acid Unknown
sodium_acetate Unknown
Sample protocol Appropriate amounts of lipid were dissolved in chloroform/methanol. Solvent was removed under nitrogen followed by at least 24 h under high vacuum. Buffer was added to yield a final lipid:buffer ratio of 50:50 wt %. Ampoules were sealed and mixed above the phase transition temperature of pure phospholipids.
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Name Value
DATE 1978
PH_METHOD buffer
ADDITIONAL_MOLECULES
Key Value
EDTA Ethylenediaminetetraacetic acid, 0.0001 M
Acetic_acid sodium acetate-acetic acid, 0.2 M
NMR
Key Value
T_RF_HEATING UNKNOWN
INSTRUMENT Bruker-Spectrospin HX-90-FT spectrometer (13.8 MHz for 2H, 36.4 MHz for 31P)
METHOD 2H:SP
SIGN_MEASURED NONE
DETAILS The experiment investigated the influence of cholesterol on the orientation and flexibility of DPPC headgroups. Deuterium quadrupole splittings and Phosphorus-31 chemical shielding anisotropies were evaluated directly from powder-type spectra. The values reported here for pure DPPC are extracted from the 0.0 cholesterol/lipid molar ratio point in Figure 2, which corresponds to measurements at 58 °C. Direct 1H-13C NMR measurements have established that the order parameters for the alpha segment are positive, while the beta segment is negative

Order Parameters DPPC

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