Experiment OP - 10.1021/bi00218a003/3

Article DOI 10.1021/bi00218a003
Data DOI N/A
Internal ID 10.1021/bi00218a003/3
Membrane composition (molar fraction)
PILPC 1-Palmitoyl-2-isolinoleoyl phosphatidylcholine 1
Solution composition Pure water
Temperature (K) 293
Total hydration 85 (mass %)
pH UNKNOWN
Reagent sources
PILPC Deuterated isolinoleoyl phosphatidylcholine lipids synthesized as described in the original publication. Lipids were purified using thin-layer chromatography and column chromatography.
H2O Deuterium-depleted water
Sample protocol Lipids were dissolved in chloroform, dried under argon, and subsequently lyophilized. Samples were hydrated with deuterium-depleted water (~200 µL for ~35 mg lipid), followed by freeze–thaw–vortex cycles to form multilamellar vesicles.
Show on GitHub experiments/OrderParameters/10.1021/bi00218a003/3 main

Name Value
PH_METHOD UNKNOWN
NMR
Key Value
INSTRUMENT Bruker MSL-300 (46.06 MHz) and custom-built spectrometer (30.7 MHz)
METHOD 2H:QE
SIGN_MEASURED NONE
T_RF_HEATING UNKNOWN
DETAILS 2H NMR quadrupolar echo experiments were performed to obtain spectra. Spectra were dePaked to extract segmental order parameters.

Order Parameters PILPC

View JSON main