| Article DOI |
10.1002/j.1460-2075.1987.tb02595.x |
| Data DOI |
N/A |
| Internal ID |
10.1002/j.1460-2075.1987.tb02595.x/2 |
| Membrane composition (molar fraction) |
| POPE |
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine |
0.099 |
| POPC |
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine |
0.901 |
|
| Solution composition |
| Compound |
Mass % |
| CLA |
0.533 |
| SOD |
0.345 |
|
| Temperature (K) |
298 |
| Total hydration |
66.7 (mass %) |
| pH |
7.4 |
| Reagent sources |
| POPC |
Choline headgroup is alpha- and beta-deuterated. POPC synthesized from corresponding PA (Avanti Polar Lipids, Birmingham, AL, USA) following the method of Harbison and Griffin (1984). Alpha- and beta-deuterated choline synthesized as described in Gally et al. (1975) and Seelig and Gally (1976).
|
| POPE |
Avanti Polar Lipids, Birmingham, AL, USA |
| H2O |
Deuterium-depleted water |
|
| Sample protocol |
Mixtures of headgroup deuterated POPC and POPE were prepared by pipetting corresponding volumes of concentrated stock solutions (10 mg/ml in dichloromethane/methanol) into NMR measuring tubes. The solvent was evaporated and lipids were dried under high vacuum (under P2O5) for several hours. Phospholipids were dispersed at room temperature in 20 mM Hepes (pH 7.4) buffer containing 150 mM NaCl, prepared with deuterium-depleted water. Lipid/buffer ratio was 1:2 (%w/%v).
|
| Show on GitHub |
experiments/OrderParameters/10.1002/j.1460-2075.1987.tb02595.x/2 main |
| Name |
Value |
| ADDITIONAL_MOLECULES |
| Key |
Value |
| HEPES |
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 20 mM |
|
| PH_METHOD |
buffer
|
| NMR |
| Key |
Value |
| INSTRUMENT |
Bruker-Spektrospin CXP 300 |
| METHOD |
2H:QE |
| SIGN_MEASURED |
NONE |
| T_RF_HEATING |
UNKNOWN |
| DETAILS |
2H NMR spectra measured using quadrupole echo technique (Davis et al., 1976), 46.1 MHz. 90 degree pulses of 3.5-4.0 us with echo separation of 30 us. 4K data points, quadrature phase detection, spectral width of 50 kHz. Recycle delay of at least 7 T1. Alpha and beta order parameters interpolated from Fig. 5A and 5B respectively at Xb=0.099 using WebPlotDigitizer. SCD = 0.00784 * delta_vQ(kHz).
|
|
No OP Data Available for POPE
Order Parameters POPC
View JSON main