Experiment FF - 10.1016/j.chemphyslip.2020.104892/2

Article DOI 10.1016/j.chemphyslip.2020.104892
Data DOI N/A
Internal ID 10.1016/j.chemphyslip.2020.104892/2
Membrane composition (molar fraction)
SDPC 1-stearyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine 1
Solution composition Pure water
Temperature (K) 303
Total hydration 99 (mass %)
pH N/A
Reagent sources
SDPC Avanti Polar Lipids
H2O High-Q purification system
Sample protocol SDPC lipid films were prepared from chloroform stock solutions and dried under Ar gas with gentle heating, followed by vacuum drying for at least 4 h. Samples were hydrated with ultrapure H2O or D2O using repeated freeze–thaw cycles. Vesicles were extruded through 50 nm polycarbonate membranes to form ULVs. Final lipid concentration was approximately 12 mg/ml. All handling was performed under an Ar atmosphere to limit lipid oxidation.
X-ray detector FLICAM CCD camera
X-ray source CHESS G-1 station
X-ray wavelength (nm) 1.18
X-ray beam size (mm) 0.24 x 0.24 mm
X-ray distance to sample (m) 0.4236
X-ray data type N/A
X-ray exposure time (s) N/A
X-ray number of frames N/A
X-ray sample type SUV
X-ray Q-range (Å⁻¹) N/A
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Name Value
DATE 2020-02-12 14:12:18